Wallace F. Marshall


Associate Professor, Department of Biochemistry & Biophysics, UCSF

Marshall Lab Home Page


Education

B.E. Electrical Engineering, SUNY Stony Brook 1991 (Advisor Velio Marsocci)
B.S. Biochemistry, SUNY Stony Brook, 1991 (Advisor Rolf Sternglanz)
Ph.D. Biochemistry, UCSF, 1997 (Advisor John Sedat)
Postdoc, MCDB Dept. Yale University, completed 2003 (Advisor Joel Rosenbaum)



Honors & Awards

2007 W.M. Keck Foundation Distinguished Young Scholar in Medical Research

2005 Searle Scholars Award

2000 Leukemia and Lymphoma Society Special Fellow

1997 Helen Hay Whitney Foundation Postdoctoral Fellow

1991 Howard Hughes Medical Institute Predoctoral Fellow




Professional Service and Society Membership

- Editorial Board, Current Biology, 2003 - present
- Advisory Board, International Review of Cytology, 2006 - present
- Headline Writer, Trends in Cell Biology, 1998-2002
- IEEE Engineering in Medicine and Biology Society Member
- American Society for Cell Biology member
- Annotation team member for Chlamydomonas Genome Project release 3.0
- Special emphasis panel member, NIH CDF-1 study section, 2004
- Grant reviewer, NIH ZGM1 BRT-0 CO panel reviewing R13 conference grant applications
- Ad hoc grant reviewer, National Science Foundation MCB division (8 grants total)
- Session Chair, International Chlamydomonas Conference, Portland, OR 2006
- Session Chair, FASEB meeting on Cilia, Saxtons River VT 2007
- Teaching Volunteer, Yale Science Education Outreach Program 2001
- Contributing author for text "Molecular Biology of the Cell" by Alberts et al.
- Organized series of pre-meeting sessions entitled "Building the Cell" on the topic of cellular morphogenesis,
held at the American Society for Cell Biology meetings in 2001, 2002, 2003, 2006, and 2007.
- Moderator and co-organizer, ASCB working group mini-symposium on "The Nature of Cytoplasm" 2007



Selected Invited Presentations (total 50)

Feb 2008 Symposium on Spatial Dynamics of Complex Systems, UC Irvine, CA
Mar 2008 Workshop on Organization of Biological Networks, Inst. of Math & Applications
Dec 2007 American Society for Cell Biology. Washington, D.C.
Sep 2007 Dept. of Systems Biology, Harvard Medical School, Boston MA
Aug 2007 FASEB meeting on Biology of Cilia. Saxtons River VT
Jul 2007 Gordon Conference on Motile & Contractile Systems. Colby-Sawyer NH
May 2007 Student Organized Symposium, MCDB/Biochemistry, CU Boulder, CO
Jul 2006 Gordon Conference on Molecular Cell Biology. Tilton, NH
Jan 2006 Replication of Structures, Cells, and Organisms. TLL, Singapore
Sep 2005 EMBO Workshop on Centrosomes, Heidelberg, Germany
May 2004 Society of French Language Protistologists meeting, Montpellier, France
Dec 2003 American Society for Cell Biology. San Francisco, CA
Feb 2003 Cell Biology of Development and Disease, TLL Singapore
Dec 2002 American Society for Cell Biology. San Francisco, CA
Aug 2002 Gordon Conference on Plant and Fungal Cytoskeleton, Andover, NH
Dec 2001 American Society for Cell Biology. Washington, DC
Aug 2000 Gordon Conference on Plant and Fungal Cytoskeleton, Andover, NH
Mar 1999 German Society for Cell Biology annual meeting. Rostock, Germany
Jul 1998 Gordon Conference on Molecular Cytogenetics, Andover, NH
Dec 1997 American Society for Cell Biology. Washington, DC
Dec 1996 American Society for Cell Biology. San Francisco, CA
Dec 1995 American Society for Cell Biology. Washington, DC



RESEARCH SUPPORT

2006-2011 Agency: National Institutes of Health Grant # 1 R01 GM077004
Project: Genetic Analysis of Centriole Duplication and Function

2007-2012 Agency: WM Keck Foundation Young Scholars Program
Project: Systems Biology of Ciliary Disease

2006-2008 Agency: National Institutes of Health Grant # 1 R03 HD051583
Project: Centriole Orientation During Left/Right Symmetry Breaking

2007-2008 Agency: National Institutes of Health Grant # 1 R03 MH081217
Project: Small Molecule Inhibitors of Cilia

2004-2007 Agency: National Science Foundation Grant # MCB 0416310
Project: Flagellar Length Control in Chlamydomonas.

2006-2008 Agency: Concern Foundation Conquer Cancer Now grant
Project: Attacking Centrioles

2005-2010 Agency: Sandler Program in Basic Sciences, Blue Sky Award
Project: How Cells Tell Direction - Manipulating the Cellular Compass



TEACHING

BC112 - Biochemistry (Pharmacy). Presentation of 5 lectures per year on the topic of lipid metabolism to students in the UCSF school of Pharmacy (as part of the Biochemistry course BC112). The lectures are presented at an undergraduate level of detail.

Cell Biology 245 (Graduate). Presentation of 4 lectures per year on the topic of the cytoskeleton to graduate students in the Tetrad program (as part of the Cell Biology course CB 245).

Bioengineering 297 (Graduate). I am responsible for teaching principles of morphological image processing and computer vision as part of the graduate bioengineering course on principles and practice of light microscopy.

Biochem 246 Developmental Biology (Graduate). I have developed a lecture for the graduate developmental biology course on the topic of self-organization and symmetry breaking. The lecture emphasizes the role of non-equilibrium thermodynamics in key developmental processes during embryogenesis.

Developmental Biology Mini-Course (Graduate). I co-organize and run a two-week intensive literature-based minicourse on the topic of spatial signaling by diffusible morphogen gradients. This mini-course is an interdisciplinary investigation into the interplay between the physics of diffusion and the role of diffusion in developmental biology, designed to allow multiple entry-points for participation by students enrolled in biochemistry, genetics, developmental biology, and biophysics programs.




Students Supervised

Ph.D. Students
Fall 2003 - present Jessica Feldman NSF & Fletcher-Jones fellowships
Winter 2004 - present Kimberly Wemmer
Summer 2004 - present Lani Keller American Heart Assoc. fellowship
Summer 2004 - present Elisa Kannegaard NSF fellowship
Summer 2006 - present Benjamin Engel
Summer 2006 - present Will Ludington NSF fellowship

Undergraduate and High School Students
Summer 2004 Roshan Karki Eastern Connecticut State University
Summer 2005 Christopher Baker Oberlin College
Summer 2006 Pedro Perez Mission High School, San Francisco
Summer 2006 Susanna Sjodin Uppsala University, Sweden

Postdoctoral Fellows Supervised
2003 - 2006 Shigenori Nonaka Ph.D. University of Tokyo
current position: Assistant Professor, National Institute of Basic Biology, Okazaki Japan
2006 - present Hiroaki Ishikawa Ph.D. Kyoto University
2006 - present Susanne Rafelski Ph.D. Stanford University
2006 - present Juliette Azimzadeh Ph.D. INRA Versailles, France




Research Publications

Marshall, W.F., Agard, D.A., and Sedat, J.W. 1994. Motion estimation and visualization for four-dimensional optical microscopy. Proc. Soc. Photo-Optical Instrumentation Engineers 2184,149-158.

Marshall, W.F., Agard, D.A., and Sedat, J.W. 1995. Quantitative analysis of chromosome motion in Drosophila melanogaster. Proc. Soc. Photo-Optical Instrumentation Engineers 2412,33-43.

Marshall, W.F., Agard, D.A., and Sedat, J.W. 1996. Chromosome mechanics in vivo: quantitative analysis of nonrigid 3D chromosome motion in Drosophila embryos. Proc. Soc. Photo-Optical Instrumentation Engineers. 2678,142-150.

Marshall, W.F., Dernburg, A.F., Harmon, B., Agard, D.A., and Sedat, J.W. 1996. Specific interactions of chromatin with the nuclear envelope: Positional determination within the nucleus in Drosophila melanogaster. Molecular Biology of the Cell 7,825-42.

Dernburg, A.F., Broman, K.W., Fung, J.C., Marshall, W.F., Philips, J., Agard, D.A., and Sedat, J.W. 1996. Perturbation of nuclear architecture by long-distance interactions. Cell 85,745-759.

Nunnari, J., Marshall, W.F., Straight, A., Murray, A., Sedat, J.W., and Walter, P. 1997. Mitochondrial transmission during mating in Saccharomyces cerevisiae is determined by mitochondrial fusion and fission and the intramitochondrial segregation of mitochondrial DNA. Molecular Biology of the Cell 8,1233-1242.

Marshall, W.F., Straight, A.F., Marko, J., Dernburg, A.F., Swedlow, J.R., Murray, A., Belmont, A., Agard, D.A., and Sedat, J.W. 1997. Interphase chromatin undergoes constrained diffusional motion in living cells. Current Biology 7,930-939.

Straight, A.F., Marshall, W.F., Sedat, J.W., and Murray, A.W. 1997. Mitosis in living budding yeast: anaphase A but no metaphase plate. Science 277,574-578.

Bass, H.W., Marshall, W.F., Sedat, J.W., Agard, D.A., and Cande, W.Z. 1997. Telomeres cluster de novo before the initiation of synapsis: a three-dimensional spatial analysis of telomere positions before and during meiotic prophase. J. Cell Biol. 137,5-18.

Oegema, K., Marshall, W.F., Sedat, J., and Alberts, B. 1997. Two proteins that cycle asynchronously between centrosomes and nuclear structures: Drosophila CP60 and CP190. J. Cell Sci. 110,1573-1583.

Fung, J.C., Marshall, W.F., Dernburg, A.F., Agard, D.A., and Sedat, J.W. 1998. Homologous chromosome pairing in Drosophila melanogaster proceeds through multiple independent initiations. J. Cell Biol. 141,5-20.

Marshall, W.F., Marko, J.F., Agard, D.A., and Sedat, J.W. 2001. Chromosome elasticity and mitotic polar ejection force measured in living Drosophila embryos using four-dimensional microscopy-based motion analysis. Curr. Biol. 11,569-578.

Marshall, W.F. and Rosenbaum, J.L. 2001. Intraflagellar transport balances continuous turnover of outer doublet microtubules: implications for flagellar length control. J. Cell Biol. 155,405-414.
- Article highlighted in "Editor's Choice" section, Science Magazine, November 2001.
- Article highlighted in "In Brief" section, Nature Rev. Molecular Cell Biol Dec. 2001.
- Article highlighted in "In This Issue" section, J. Cell Biol. October 2001.

Marshall, W.F., Vucica, Y, and Rosenbaum, J.L. 2001. Kinetics and regulation of de novo centriole assembly: implications for the mechanism of centriole duplication. Curr. Biol. 11,308-317.

Zamora, I., Feldman, J.L., and Marshall, W.F. 2004. PCR-based assay for mating type and diploidy in Chlamydomonas reinhardtii. Biotechniques. 37,534-535.

Marshall, W.F. 2004. Human ciliary proteome contains a homolog of the zebrafish polycystic kidney disease gene qilin. Curr. Biol. 14,R913-914.

Keller, L.C., Romijn, E.P., Zamora, I., Yates, J.R. 3rd., and Marshall, W.F. 2005. Proteomic analysis of isolated Chlamydomonas centrioles reveals orthologs of ciliary disease genes. Curr. Biol. 15,1090-8.
-Article recommended by Faculty of 1000 web site.

Stolc, V., Samanta, M.P., Tongprasit, W., and Marshall, W.F. 2005. Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes. Proc. Natl. Acad. Sci. U.S.A. 102,3703-3707.

Marshall, W.F., Qin, H., Rodrigo Brenni, M., and Rosenbaum, J.L. 2005. Flagellar length control system: testing a simple model based on intraflagellar transport and turnover. Mol. Biol. Cell 16,270-278.
- Article highlighted in "Incytes" section of ASCB Newsletter, January 2005 issue.

Zamora, I., and Marshall, W.F. 2005. A mutation in the centriole-associated protein centrin causes genomic instability via increased chromosome loss. BMC Biology. 3,15.

Nonaka, S., Yoshiba, S., Goto, T., Marshall, W.F., and Hamada, H. 2005. De novo formation of left-right asymmetry by posterior tilt of nodal cilia. PloS Biology. 3,e268
-Article rated as ÒMust ReadÓ by Faculty of 1000 web site.

Kennedy, T.E., Wang, H., Marshall, W.F., and Tessier-Lavigne, M. 2006. Axon guidance by diffusible chemoattractants: a gradient of netrin protein in the developing spinal cord. J. Neurosci. 26,8866-74.

Feldman, J.L, Geimer, S., and Marshall, W.F. 2007. The mother centriole plays an instructive role in defining cell geometry. PLoS Biology. 5,e149.
- Article selected as "Editors Pick"
- Article featured in "Research Roundup" section Journal of Cell Biology 177, 946.

Marshall, W.F. 2007. Stability and robustness of an organelle number control system: modeling and measuring homeostatic regulation of centriole abundance. Biophysical Journal. 93,1818-33.

Merchant, S., Prochnik, S., Vallon, O., Harris, E.H., Karpowicz, S.J., Witman, G.B., Terry, A., Salamov, A., Fritz-Laylin, L.K., Marechal-Drouard, L., Marshall, W.F., Qu, L.H., Nelson, D.R, et al. 2007. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 318,245-50.
- Article rated "Must Read" by Faculty of 1000 web site.




Selected Review Articles & Book Chapters (total of 26)

Marshall, W.F., Fung, J.C., and Sedat, J. 1997. Deconstructing the nucleus: global organization from local interactions. Curr. Opin. Gen. Devel. 7,2259-263.

Marshall, W.F. 2001. Centrioles take center stage. Curr. Biol. 11,R487-R496.

Marshall, W.F. 2002. Order & disorder in the nucleus. Curr. Biol. 12,R185-R192.

Marshall, W.F. 2002. Size control in dynamic organelles. Trends Cell Biol. 12,414-419.

Marshall, W.F. 2003. Gene expression and nuclear architecture during development and differentiation. Mech. Development 120,1217-1230.

Marshall, W.F. 2004. Cellular length control systems. Ann. Rev. Cell Dev. Biol. 20,677-93.

Mogilner, A., Wollman, R., and Marshall, W.F. 2006. Quantitative modeling in cell biology: what is it good for? Dev. Cell 11,1-9.
- Article rated "Must Read" by Faculty of 1000 web site

Marshall, W.F. and Nonaka, S. 2006. Cilia: tuning in to the cell's antennae. Curr. Biol. 16,R604-R614.

Marshall, W.F. 2007. Immotile cilia syndrome. In Encyclopedia of Molecular Mechanisms of Disease, M. Bilic, editor. Springer-Verlag, Heidelberg, Germany. in press.

Marshall, W.F. 2007. Use of transcriptomic data to support organelle proteomic analysis. In Organelle Proteomics, D. Pfleiger and J. Rossier, editors. Humana Press, Totowa NJ. in press.

Wemmer, K. and Marshall, W.F. 2007. Flagellar length control in Chlamydomonas - A paradigm for organelle size regulation. Int. Rev. Cytol. 260,175-212.

Marshall, W.F. 2007. What is the function of centrioles? J. Cell. Biochem. 100,916-22.

Marshall, W.F. and Kintner, C. 2007. Cilia orientation and the fluid mechanics of development. Curr. Opin. Cell Biol. 20, 48-52.

Marshall, W.F. 2007. Centriole assembly: the origin of nine-ness. Curr. Biol. 17, R1057-9.

Marshall, W.F. 2007. The cell biological basis of ciliary disease. J. Cell Biol. 180, 17-21.